The reason that most pregnancy tests recommend first morning urine is that this urine typically contains the highest concentration of hCG, the pregnancy hormone. However, if you work nights or your sleep is interrupted by bathroom breaks, you may not have the most concentrated urine at this time.
You can recreate first morning urine by not using the bathroom for at least four hours. You must also not drink more than your normal amount of water or else you will dilute your urine. It does not matter if you urinate on the stick or in a cup.
Am I Pregnant?
Typically after the fourth of fifth week of pregnancy your levels of hCG are high enough without having to use first morning urine.
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